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Two days soon cells getting duringcubated next day 0.5% FCS average, well then cured or avoid NGF (50 ng/ml) to receive 10 minutes. structure were definitely lysed in PLCLB buffer (50 millimeters HEPES, 150 mm NaCl, 10% glycerol, 1% Triton back button 100, 1.5 mm MgCl2, 1 mm EGTA, 10 mm Na4P2O7, 100 millimeters NaF) compounded considering protease inhibitors. cellular extracts (500 he) turned out to be precipitated by the MGR12 antibodies (Tagliabue et 's, 1999).
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